Coding

Part:BBa_K1321090:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-07)

Phytochelatin (PC) EC20 + Linker-dCBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

dCBD is EnLinker-CBH2-EnLinker-CBH1 from T.reesei (BBa_K1321340). Please refer to BBa_K1321005 for detailed information on synthetic phytochelatin EC(20). Both dCBD and the phytochelatin were designed and ordered from GeneArt® Gene (Invitrogen™ Life Technologies).

References